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首页 >> 实验室试剂 >> 生命科学 >> Recombinant Human Alkaline Phosphatase/ALPL Protein
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  • 所属类别:实验室试剂
  • 产品品牌:阿拉丁
  • 价格区间:1万以上
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阿拉丁rp183653


Recombinant Human Alkaline Phosphatase/ALPL Protein

别名重组人Alkaline Phosphatase/ALPL蛋白 | 重组人非组织特异性碱性磷酸酶蛋白
英文别名Akp2 | Alkaline phosphatase liver/bone/kidney isozyme | alkaline phosphatase, liver/bone/kidney | alkaline phosphatase, tissue-nonspecific isozyme | alkaline phosphomonoesterase | ALPL | APTNAP | AP-TNAP | EC 3.1.3.1 | FLJ40094 | FLJ93059 | glycerophospha
规格或纯度无动物源, 无载体, 生物活性, ActiBioPure™, ≥95%(SDS-PAGE), 见COA
生化机理Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization. Post-translational: N-glycosylated.
生物活性Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP) (M103814). The specific activity is >30,000 pmol/min/µg.
内毒素水平<1.0 EU/μg
表达系统HEK293
种属人(Human)
氨基酸1-502
序列MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSHHHHHH
蛋白标签C-His
无载体Yes
无动物源Yes
Accession #P05186
来源重组表达
预测分子量54.2 kDa
SDS-PAGE59.1-72.1 kDa, under reducing conditions; 57.7-70.8 kDa, under non-reducing conditions.


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