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  • 5-DBCO-PEG4-dUTP
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  • 5-DBCO-PEG4-dUTP

    实验室试剂
    阿拉丁
    2000-5000
    • ¥3999.90
      ¥0.00
      ¥3999.90
      ¥2399.94
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商品参数
  • 所属类别:实验室试剂
  • 产品品牌:阿拉丁
  • 价格区间:2000-5000
商品描述


品牌货号


英文别名

阿拉丁D487788


5-DBCO-PEG₄-dUTP

规格或纯度≥95%, 10mM solution in 100 mM Tris-HCl
英文名称5-DBCO-PEG4-dUTP
储存温度-20°C储存
运输条件超低温冰袋运输
产品介绍


5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice
to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
to crosslink the DNA to Azide-functionalized biomolecules 
e.g.proteins


Applications:
Incorporation into DNA/cDNA by
PCR with Taq polymerase in-house data


pH: 7.5 ±0.5
Spectroscopic Properties: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)


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