新一代的阳离子聚合物转染试剂，具有高效率转染所必备特征，细胞毒性低。与其它转染试剂相比，本品很稳定，不被血清清除，使得基因转染的操作简便易行，重复性好。本产品已被成功应用于很多原代培养细胞和转化细胞株的基因转染，在国内实验室广泛使用。
特性 转染效率高,在有与没有血清存在的细胞培养基中均能获得高效率转染； 细胞毒性低适用于众多原代培养细胞/转化细胞株转染,瞬时/稳定转染,贴壁/悬浮细胞转染； 转染程序简单，转染实验可以在半小时内完成。
运输贮存 5.0mg/ml 溶液，请4℃保存，切勿冻存。
本品成功转染的部分细胞株
| 细胞株 | 来源 | 细胞类型 | | HEK 293 | Human | 胚胎肾细胞（ Embryonic kidney ） | | HeLa | Human | 子宫颈癌细胞（ Cervix carcinoma ） | | NIH 3T3 | Mouse | 胚胎纤维原细胞（ Embryo fibroblast ） | | BNL CL2 | Mouse | 胚胎肾细胞（ Embryonic kidney cells ） | | HepG2 | Human | 肝癌细胞（ Hepatocarcinoma ） | | COS7 | Monkey | 猿猴病毒 40 肝脏转化细胞（ SV40 liver transformed ） | | CHO | Chinese hamster | 中国仓鼠卵巢细胞（ Ovary ） | | SHSY-5Y | Human | 成神经细胞瘤细胞（ Neuroblastoma ） | | IMR 32 | Human | 成神经细胞瘤细胞（ Neuroblastoma ） | | MRC5 | Human | 胎儿肺上皮细胞（ Fetal lung epithelium ） | | MCF7 | Human | 乳腺癌细胞（ Breast adenocarcinoma ） | | K562 | Human | 慢性白血病细胞（ Chronic leukemia ） | | SKOV-3 | Human | 卵巢腺癌细胞（ Ovarian adenocarcinoma ） | | IGROV-1 | Human | 卵巢腺癌细胞（ Ovarian adenocarcinoma ） | | HUV-EC(primary cell) | Huamn | 脐带静脉内皮状细胞 （Umbilical vein endothelial cell ） | | C6 | Rat | 脑胶质瘤细胞 (Glioma C6 cell) | | Primary cell | Rat | 新生大鼠心肌细胞（ Neonatal Rat Cardiomyocyte ） | | SGC-7901 | Human | 胃腺癌细胞 （ Gastric cancer ） | | Eca-109 | Human | 食管癌细胞 （ Esophageal carcinoma ） | | SK-N-SH | Human | 神经母细胞瘤细胞（ Neuroblastoma ） | | ZR-75-1 | Human | 乳腺癌细胞（ Breast cancer ） | | THP-1 | Human | 单核细胞白血病（ Monocyte ） | | WEHI231 | Mouse | B 淋巴细胞 （ Immature B ） | | SK-HEP-1 | Human | 肝癌细胞（ Liver adenocarcinoma ） | | P815 | Mouse | 肥大细胞癌细胞（ Mastocytoma ） | | BGC-823 | Human | 胃腺癌细胞（ Gastric carcinoma ） | | CV-1 | African green monkey | 非洲绿猴肾细胞（ Kidney ） | | SiHa | Human | 宫颈癌细胞（ Cervical carcinoma ） | | A549 | Human | 肺癌细胞 （ Lung carcinoma ） | | MDA-MB-231 | Human | 乳腺癌细胞（ Breast adenocarcinoma ） | | T-47D | Human | 乳腺管癌细胞 （ Breast cancer ） | | U-937 | Human | 组织细胞淋巴瘤细胞（ Histiocytic lymphoma ） | | HuH-7 | Human | 肝癌细胞（ Hepatoma ） | | C929 | Human | 骨髄性白血病（ Bbone marrow ） | | XG-1 | Human | 多发性骨髓瘤细胞 （ Myeloma ） | | MC3T3-E1 | Rat | 成骨样细胞 （ Osteogenic ） | | Hep-2 | Human | 喉癌细胞（ Larynx carcinoma squamou ） | | MDCK | Dog | 肾细胞 Kidney （ Madin-Darby ） | | BMSC | Mouse | 大鼠骨髓间充质干细胞（ Bone marrow stromal ） | | BEL-7402 | Human | 肝癌细胞（ Hhepatic carcinoma ） | | 2BS | Human | 人胚肺二倍体成纤维细胞（ Diploid fibroblast ） | | U373 | Human | 星状细胞瘤 （ Glioblastoma astrocytoma ） |
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A new generation of cationic polymer transfection reagent, It has the characteristics of high transfection efficiency and low cytotoxicity. Compared with other transfection reagents, this product was more effective ® It is stable and can not be eliminated by serum, which makes gene transfection easy and repeatable.It has been successfully used in gene transfection of many primary cultured cells and transformed cell lines. It is widely used in domestic laboratories.
Characteristic The transfection efficiency was high, and high transfection efficiency could be obtained in cell culture medium with or without serum; Low cytotoxicity, suitable for many primary culture cells / transformed cell lines transfection, transient / stable transfection, adherent / suspension cell transfection; The transfection procedure is simple, and the transfection experiment can be completed in half an hour.
Transportation and storage 5.0 mg/ml solution, please store at 4 ℃, do not freeze.
Some cell lines were successfully transfected by this transfection reagent
| cell line | source | Cell type | | HEK 293 | Human | Embryonic kidney | | HeLa | Human | Cervix carcinoma | | NIH 3T3 | Mouse | Embryo fibroblast | | BNL CL2 | Mouse | Embryonic kidney cells | | HepG2 | Human | Hepatocarcinoma | | COS7 | Monkey | SV40 liver transformed | | CHO | Chinese hamster | Ovary | | SHSY-5Y | Human | Neuroblastoma | | IMR 32 | Human | Neuroblastoma | | MRC5 | Human | Fetal lung epithelium | | MCF7 | Human | Breast adenocarcinoma | | K562 | Human | Chronic leukemia | | SKOV-3 | Human | Ovarian adenocarcinoma | | IGROV-1 | Human | Ovarian adenocarcinoma | | HUV-EC(primary cell) | Huamn | Umbilical vein endothelial cell | | C6 | Rat | Glioma C6 cell | | Primary cell | Rat | Neonatal Rat Cardiomyocyte | | SGC-7901 | Human | Gastric cancer | | Eca-109 | Human | Esophageal carcinoma | | SK-N-SH | Human | Neuroblastoma | | ZR-75-1 | Human | Breast cancer | | THP-1 | Human | Monocyte | | WEHI231 | Mouse | Immature B | | SK-HEP-1 | Human | Liver adenocarcinoma | | P815 | Mouse | Mastocytoma | | BGC-823 | Human | Gastric carcinoma | | CV-1 | African green monkey | Kidney | | SiHa | Human | Cervical carcinoma | | A549 | Human | Lung carcinoma | | MDA-MB-231 | Human | Breast adenocarcinoma | | T-47D | Human | Breast cancer | | U-937 | Human | Histiocytic lymphoma | | HuH-7 | Human | Hepatoma | | C929 | Human | Bbone marrow | | XG-1 | Human | Myeloma | | MC3T3-E1 | Rat | Osteogenic | | Hep-2 | Human | Larynx carcinoma squamou | | MDCK | Dog | Kidney Madin-Darby | | BMSC | Mouse | Bone marrow stromal | | BEL-7402 | Human | Hhepatic carcinoma | | 2BS | Human | Diploid fibroblast | | U373 | Human | Glioblastoma astrocytoma |
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