UltraBio™Insect转染试剂(UltraBio™Insect Transfection Reagent)是一种基于阳离子脂质体等最新转染技术的非常高效的新型昆虫细胞转染试剂。本产品转染效率高、操作简单、细胞毒性低、重复性好，达到了国际最主流昆虫细胞转染试剂的转染效果。本产品适用于将pFastBac1等杆状病毒表达载体质粒DNA转染到Sf9、Sf21和High Five等昆虫细胞以用于Bac-to-Bac、BaculoDirect和InsectSelect表达系统的蛋白表达。UltraBio™Insect转染试剂专门针对昆虫细胞进行了多方面的优化，转染效率非常高，对昆虫细胞Sf9的实测阳性率可达98%以上(参考图1)。常规的脂质体细胞转染试剂如UltraBio™6000转染试剂、UltraBio™2000 Reagent等都是无法顺利转染昆虫细胞的，必须使用昆虫细胞专用的转染试剂。图1.UltraBio™Insect转染试剂用pFastBac1-EGFP质粒转化DH10Bac菌株后抽提获得的bacmid转染Sf9细胞96小时后的实拍效果图。A. 明场照片；B. 荧光照片。UltraBio™Insect转染试剂操作简单，使用方法和常用的Cellfectin II Reagent完全一致。UltraBio™Insect转染试剂转染过表达质粒后，通常96小时后达到较高的蛋白表达水平。UltraBio™Insect转染试剂的转染效果可以通过转染表达EGFP等荧光蛋白的bacmid进行快速鉴定。关于昆虫细胞系统的蛋白表达与纯化的详细介绍，请见如下网页： http://www.aladdin-e.com。 如果用于六孔板的细胞转染，一个包装的C0551-0.5ml转染试剂大约可以转染60个孔，一个包装的C0551-1.5ml转染试剂大约可以转染180个孔，一个包装的C0551-7.5ml转染试剂大约可以转染900个孔。

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Aladdin's UltraBio™Insect Transfection Reagent is a novel, cationic liposome-mediated transfection formulation developed for highly efficient transfection of DNA into insect cells, with good consistency and extremely low cytotoxicity. It can be used to transfect baculovirus plasmid (bacmid) DNA, such as pFastBac1, into insect cells such as Sf9, Sf12, and High Five™, facilitating the expression of target genes with Bac-to-Bac®, BaculoDirect™, and InsectSelect™ expression systems.UltraBio™Insect Transfection Reagent is optimized specifically for high-performance transfection of insect cells, with a transfection efficiency of over 98% in Sf9 cells (Figure 1). Please note that the conventional liposome transfection reagents, such as UltraBio™6000 transfection Reagent and UltraBio™2000 Reagent, are unable to transfect insect cells effectively.Figure 1. Sf9 insect cells transfected with EGFP recombinant bacmid using Aladdin's UltraBio™Insect Transfection Reagent . pFastBac-GFP plasmid was transformed into DH10Bac strain to obtain EGFP recombinant bacmid which was then transfected into Sf9 insect cells. Cells were harvested and observed in the bright field (A) and the fluorescence channel (B) under fluorescence microscopy 96 hours after transfection. UltraBio™Insect Transfection Reagent is easy to use. Its transfection protocol is the same as the commonly used Cellfectin® II Reagent.Generally, it takes at least 96 hours after transfection of expression plasmid to yield high protein expression levels in insect cells.The transfection efficiency can be determined by transfecting bacmid carrying genes encoding fluorescent proteins, such as EGFP.For insect cells cultured in 6-well plates, C0551-0.5ml is sufficient for 60 transfections, C0551-1.5ml is sufficient for 180 transfections, and C0551-7.5ml is sufficient for 900 transfections.