EMSA/Gel-Shift结合缓冲液(5X)(EMSA/Gel-Shift Binding Buffer,5X)是用于EMSA/Gel-Shift的结合缓冲液。通过EMSA，也称gel-shift，可以研究目的蛋白和特定的DNA序列的结合情况，从而可以研究细胞内一些转录因子的激活水平。EMSA/Gel-Shift 结合缓冲液(5X)可以用于EMSA研究时细胞核蛋白或纯化的转录因子和特定的双链寡核苷酸的结合反应。EMSA/Gel-Shift结合缓冲液(5X)中含有poly(dI-dC)，DTT，甘油，EDTA，氯化钠，氯化镁以及Tris等有效成分。其中poly(dI-dC)的浓度经过优化，可以很好的消除蛋白和标记探针间的非特异性结合，同时又不会减弱目的转录因子和标记探针间的结合。本EMSA/Gel-Shift结合缓冲液(5X)不仅适用于同位素标记的探针，也适用于地高辛(digoxin)或生物素(biotin)标记的探针。每个包装的EMSA/Gel-Shift结合缓冲液(5X)，足够进行100个蛋白和探针的结合反应。

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EMSA/Gel-Shift Binding Buffer (5X) is the binding buffer for EMSA/Gel-Shift assay which is generally used to study protein-DNA interactions and to detect the activation level of transcription factors in cells. EMSA/Gel-Shift Binding Buffer (5X) can be used in EMSA for the binding of nuclear proteins or purified transcription factors to specific double-stranded oligonucleotides.This product contains active ingredients such as poly(dI-dC), DTT, glycerol, EDTA, NaCl, MgCl2 and Tris. The concentration of poly(dI-dC) has been optimized to eliminate non-specific bindings without affecting the specific binding between target transcription factor and the labeled probe.This product is not only suitable for isotope-labeled probes, but also for digoxin or biotin-labeled probes.This product is sufficient for 100 binding reactions between protein and probe.