品牌货号:阿拉丁T755932
产品名称 | 胰蛋白酶 来源于牛胰腺 |
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规格或纯度 | ActiBioPure™, 生物活性, GMP, 高性能, EnzymoPure™, >4000 USP U/mg powder |
生化机理 | Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity. Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin. |
产品介绍 | 胰蛋白酶是一种丝氨酸蛋白水解酶。胰蛋白酶特异性严密,专一作用于由碱性氨基酸精氨酸及亮氨酸所组成的肽键。易溶于水,不溶于三氯甲烷、乙醇,乙醚和甘油,Ca2+对胰蛋白酶有保护和激活作用。 |
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