品牌货号 规格或纯度 | 阿拉丁R751626 50X |
---|---|
稳定性与储存 | 4℃避光保存,至少1年有效。长期不使用时,也可以-20℃避光保存。 |
英文名称 | UltraBio™ Red Fluorescent DNA Marker Dye |
储存温度 | 2-8°C储存,避光 |
运输条件 | 冰袋运输 |
产品介绍 | 阿拉丁的UltraBio™红色荧光DNA Marker染料(50X),即UltraBio™ Red Fluorescent DNA Marker Dye (50X)是一种浓缩型的高安全性高灵敏度的荧光染料NA-Red (Nucleic Acid Red),专为已经添加了上样缓冲液的DNA marker设计,按照1:50的比例直接加入到已经添加了上样缓冲液的DNA marker中混匀即可使用。添加了本产品的DNA marker,无须在凝胶配制时再加入任何核酸染料或者在电泳后进行染色,上样前已经过红色荧光染料的预染,使用特别便捷,而且配制以后可以长期保存。对于不含上样缓冲液的DNAmarker,推荐使用D0076UltraBio™红色荧光DNA上样缓冲液(6X,溴酚蓝)、D0081UltraBio™红色荧光DNA上样缓冲液(6X, Red)或者UltraBio™系列的绿色荧光DNA上样缓冲液。本产品使用的染料NA-Red (Nucleic Acid Red),安全性高,致突变性极低且检测不到显著的细胞毒性。使用本产品后在300nm左右波长下检测呈现红色荧光,与EB的激发光和发射光都比较接近,无需改变滤光片及观察装置,适用于原先使用EB为染料的凝胶成像系统。本产品可用于10ml DNA marker的红色荧光预染。 注意事项: 本产品作为荧光染料须避光保存,但在使用过程中的常规室内光照不会影响其使用效果。本产品可直接加入DNA Ladder中,进行上样和电泳。电泳之后的凝胶不建议重复使用。电泳后的凝胶通常直接使用凝胶成像设备进行观察即可。如果发现背景偏高,也可以使用不含核酸酶的水洗涤背景。如果观察到条带弥散或者分离不理想,请尝试以下方法:使用新鲜配制的电泳缓冲液、降低核酸的上样量、降低琼脂糖浓度、选用更长的凝胶、降低电泳电压一倍以上并延长凝胶电泳时间以改善电泳效果、使用更薄的梳齿等。本产品兼容常用的电泳缓冲液,例如TAE和TBE。本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。为了您的安全和健康,请穿实验服并戴一次性手套操作。使用说明: 1.DNA marker中加入染料:按照每49微升已经添加了上样缓冲液的DNA marker加入1微升本产品的比例,混匀DNA marker和InstantView™红色荧光DNA Marker染料(50X),取适量的体积进行电泳。注:加入本产品后的DNA marker,须避光保存。具体的保存温度和有效期参考DNA marker的保存条件。2.电泳后,推荐使用凝胶成像仪进行观察或拍照。Aladdin's UltraBio™ Red Fluorescent DNA Marker Dye (50X) contains a concentrated, fluorescent dye, NA-Red (Nucleic Acid Red), to stain DNA after mixing with DNA markers (with loading buffer) at a ratio of 1 Precautions: As a fluorescent dye, this product should be stored in the dark. Regular indoor light during use will not affect its performance.This product can be added directly into DNA Ladder for gel loading and electrophoresis. It is not recommended to reuse the gels after electrophoresis.Gels after electrophoresis can usually be observed directly with a gel imaging system. If the background is too high, wash gels with nuclease-free water.If DNA smear is observed or DNA could not be resolved well, we recommend gel staining after electrophoresis to determine whether the problem is due to the dye. If the problem still exists, other attempts can be tried, such as using freshly prepared buffer, reducing the amount of nucleic acid loaded, reducing the concentration of dye or agarose, using a longer gel, reducing the voltage for electrophoresis and extending the electrophoresis time.This product is compatible with commonly used electrophoresis buffers, such as TAE and TBE.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use: 1. Adding dye to DNA marker: Mix this product with DNA marker at a ratio of 1:49 (e.g., add 1μl of this product into 49μl of DNA marker with the loading buffer added), and then load an appropriate volume for electrophoresis.Note: After addition of this product, the DNA marker should be stored in the dark at appropriate temperatures as required by the DNA marker.2. After electrophoresis, examine gels and take pictures by a gel imager. |
公司名称:探究者科学器材(江苏)有限公司
全国客服热线:4006-765-543
地址:江苏省宿迁市经济技术开发区富民大道88号A12栋
邮编:223800
咨询/订货
关于探究者
售后服务
会员中心
本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断、治疗或实验,不可药用,不可食用。
订购指南
Copyright © 2025 探究者科学器材(江苏)有限公司 All Rights Reserved 苏ICP备2025173400号-1