阿拉丁的UltraBio™绿色荧光DNA上样缓冲液(6X, Red) (UltraBio™ Green Fluorescent DNA Loading Buffer, 6X with Red)是一种添加了高安全性绿色荧光染料NA-Green (Nucleic Acid Green)的DNA上样缓冲液，用于琼脂糖凝胶电泳时DNA、RNA等核酸样品的荧光染色和上样，核酸的荧光染色可以在与上样缓冲液混合的瞬间完成。本产品使用便捷，染色上样一步完成：本产品中NA-Green的浓度经过优化，使用时直接加入适量本产品到核酸样品中混匀即可进行上样和电泳，无须在凝胶配制时加入任何核酸染料或者在电泳后进行染色，上样、染色同步进行，使用特别便捷。本产品安全、灵敏度高、稳定性好：本产品中使用的NA-Green具有安全(致突变性极低且检测不到显著的细胞毒性)、灵敏度高(可检测低至0.6ng的DNA)、稳定性好(热稳定性和光稳定性好)等优点。本产品的灵敏度效果参考图1。图1.不同量DNA样品使用本产品的效果图。实际效果会因DNA质量、检测仪器等的不同而存在差异，图中数据仅供参考。本产品可以使用和SYBR Green或EB相同的荧光检测体系：使用本产品染色后在约500nm波长蓝光(也可以使用约260nm紫外光)激发呈现绿色荧光，适用于原先使用SYBR Green或SYBR Gold为核酸染料的凝胶成像和检测系统，例如对人体无害的蓝光灯或蓝光成像仪。对于原来用于EB染料观察的系统，可以考虑选用阿拉丁的使用NA-Red染料的D0076UltraBio™红色荧光DNA上样缓冲液(6X, 溴酚蓝)或D0081 UltraBio™红色荧光DNA上样缓冲液(6X, Red)，但也可以使用本产品。使用本产品并且用EB的紫外检测体系时，检测灵敏度会比使用NA-Red染料的产品低约4-5倍。对于既可以观察EB也可以观察SYBR Green的具有紫外和蓝光两种激发光的凝胶成像系统，则推荐直接使用本产品。使用本产品时，宜配套使用D0116S/D0118S/D0121S/D0123S UltraBio™绿色荧光DNA Ladder系列产品，或者把已有的添加了常规上样缓冲液的DNA ladder和D0153 UltraBio™绿色荧光DNA Marker染料(50X)配套使用。本产品和DNA ladder配套使用的效果图参考图2。图2.不同长度的PCR产物样品使用本产品，并使用UltraBio™绿色荧光DNA Ladder 的电泳效果图。本上样缓冲液以Red为指示剂，稀释至1X后比重仍然较大，上样便捷、极易下沉，且颜色清晰可见。Red的电泳位置在相当于20-50bp的DNA片段，这样就可以完全消除染料对小片段DNA观察时的干扰。如果待上样的样品的体积为10微升，则每个样品需使用2微升本产品，本产品共可以进行500次或5000次的染色和上样。

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Aladdin's UltraBio™ Green Fluorescent DNA Loading Buffer (6X, Red) is used for loading DNA samples onto agarose or polyacrylamide gels for gel electrophoresis. It contains NA-Green(Nucleic Acid Green) fluorescent dye that binds to nucleic acids at the moment of mixing and emits Green fluorescence.This product is easy to use. A simple three-step procedure includes adding 1/6 volume of 6X DNA Loading Buffer to DNA, loading onto gel and electrophoresis. There is no need to add any dye in agarose gels or stain gels after electrophoresis.This product is safe, sensitive, and highly stable. NA-Green is a significantly safer alternative to ethidium bromide (EB), with very low mutagenicity and no cytotoxicity. It allows the detection of ＞0.6ng of DNA, and has good thermal and photo-stability. Refer to Figure 1 for the sensitivity of this product.Figure 1. Image of different amounts of DNA loaded with Aladdin's UltraBio™ Green Fluorescent DNA Loading Buffer (6X, Red) on agarose gel after electrophoresis. The actual results may vary due to differences in DNA quality and detection instrument used.Fluorescence detection system for SYBR Green or EB can be used for this product. Green fluorescence is detected on DNA samples stained by this product using blue light at a wavelength of about 500nm or UV light at 260nm. If using the detection system for EB dye, UltraBio™ Red Fluorescent DNA Loading Buffers containing NA-Red dye are recommended, although this product containing NA-Green can also be used, with a 4-5 times lower detection sensitivity. For Gel Imaging Systems with filters for both EB and SYBR Green, this product is recommended.This product uses Red as an indicator for easy visual tracking of DNA migration during electrophoresis. Red comigrates with 20-50 bp DNA fragments, which usually does not interfere with the observation of DNA fragments.UltraBio™™ Green Fluorescent DNA Ladders should be used in parallel with this product. You can also use the regular DNA ladder supplemented with an appropriate amount of Aladdin's UltraBio™ Green Fluorescent DNA Marker dye (50X). Please refer to Figure 2 for the stain result of DNA samples loaded with this product and the UltraBio™ Green Fluorescent DNA Ladder .Figure 2. Image of the UltraBio™ Green Fluorescent DNA Ladder and DNA samples in different lengths loaded with Aladdin's UltraBio™ Green Fluorescent DNA Loading Buffer (6X, Red) on agarose gel after electrophoresis.