产品介绍：
GelRed是一款新型核酸染料。这种独特的油性大分子，不易挥发升华、不易吸入人体，不能穿透细胞膜进入活体细胞内，且在凝胶染色浓度下没有诱变性，具有使用安全、检测灵敏等特点，可以作为各种核酸电泳的染色剂，适用于各种片段大小染色，它是EB的完美替代品。DNA上样缓冲液（5X, GelRed）需要和样品混合，点染使用。
组成成分：10mMTris-HCl，5mMEDTA，pH7.6，0.03% 溴酚蓝，0.03%二甲苯青，30%甘油，GelRed核酸染料。
使用方法：
1. 配合阿拉丁的DNA Marker使用。
2. 样品4μl(<500ng)与DNA上样缓冲液（5X, GelRed）1μL混匀，室温放置2分钟后点样
3. 建议用1.0-2.0% Agarose，电压4-10v/cm，1xTAE(优先选用)或0.5xTBE缓冲液电泳。注意及时更换电泳缓冲液并使用新制备的凝胶，以达到理想的结果。
4. 琼脂糖凝胶中不需要加任何染料，电泳后可直接在紫外灯下观察电泳条带。染料兼容常用的紫外灯。

Product Introduction:
GelRed is a novel nucleic acid stain. This unique oil-soluble macromolecule is non-volatile, non-inhalable, and cannot penetrate cell membranes to enter living cells. Additionally, it exhibits no mutagenicity at the gel-staining concentration. GelRed is characterized by its safety in use and high sensitivity in detection. It can be used as a staining agent for various nucleic acid electrophoresis applications and is suitable for staining nucleic acids of all fragment sizes. GelRed is an ideal alternative to ethidium bromide (EB). DNA Loading Buffer (5X, GelRed) needs to be mixed with the sample for staining.
Composition: 10 mM Tris-HCl, 5 mM EDTA, pH 7.6, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, GelRed Nucleic Acid Stain.
Usage Instructions:
1. Use in combination with Aladdin DNA Marker.
2. Mix 4 μl of sample (<500 ng) with 1 μl of DNA Loading Buffer (5X, GelRed). Allow the mixture to stand at room temperature for 2 minutes before loading.
3. It is recommended to use 1.0-2.0% agarose gel, with a voltage of 4-10 V/cm. Perform electrophoresis using 1x TAE (preferred) or 0.5x TBE buffer. Note: Replace the electrophoresis buffer promptly and use freshly prepared gels to achieve optimal results.
4. No additional stains are required in the agarose gel. After electrophoresis, the bands can be directly visualized under UV light. The stain is compatible with commonly used UV transilluminators.