100bp DNA Ladder是由11条线状双链DNA条带组成，适用于琼脂糖凝胶电泳中100 bp-1500 bp范围内准确判断DNA片段大小，不建议用于Acrylamide凝胶电泳。本产品为即用型产品，已含有1×Loading Buffer，可根据实验需要，直接上样电泳，使用方便，电泳图像清晰。其中500 bp 条带显示为亮带

应用范围：

DNA Ladder

注意事项：
1. 使用前请将产品瞬时离心至管底，再进行后续实验。
2. 请及时更换电泳缓冲液并使用新制的凝胶，以免影响电泳结果。
3. 避免反复冻融。

使用方法：
1. 可搭配 GelstainRed 进行电泳，取5 µL加入琼脂糖凝胶的加样孔中，进行电泳。
2. 建议电泳条件为2-3%琼脂糖凝胶，电压4-10V/cm。
3. 通过GelstainRed或其他染料进行染色，观察电泳条带。

产品参数：

条带组成：100 bp，200 bp，300 bp，400 bp，500 bp，600bp，700 bp，800 bp，900 bp，1000 bp，1500 bp。

The 100bp DNA ladder is composed of 11 linear double stranded DNA bands, which is suitable for accurately judging the size of DNA fragments in the range of 100 bp-1500 BP in agarose gel electrophoresis, and is not recommended for acrylamide gel electrophoresis. This product is ready to use and contains 1 × Loading buffer can directly load samples for electrophoresis according to experimental needs, which is convenient to use and has clear electrophoresis images. Among them, the 500 BP band is shown as a bright band

Recommended electrophoretic buffer:

Recommended electrophoresis buffer：TBE

Matters needing attention：
1.Please instantaneously centrifuge the product to the bottom of the tube before use, and then carry out subsequent experiments.
2.Please replace the electrophoresis buffer in time and use the new gel to avoid affecting the electrophoresis results.
3.Avoid repeated freezing and thawing.

Instruction：
1.Electrophoresis can be carried out with GelstainRed, and 5μL is added to the sample hole of agarose gel for electrophoresis.
2.It is recommended that the electrophoresis conditions are 2-3 % agarose gel, voltage 4-10V / cm.
3.Staining with GelstainRed or other dyes to observe the electrophoretic bands.

Product parameters:

Ribbon composition：100 bp，200 bp，300 bp，400 bp，500 bp，600bp，700 bp，800 bp，900 bp，1000 bp，1500 bp

Scope of application：

DNA ladder