100 bp Ladder由9条DNA片段组成，分别为1,500 bp、1,000 bp、800 bp、600 bp、500 bp、400 bp、300 bp、200 bp和100 bp。本产品为已含有1×Loading Buffer的DNA溶液，可取5 μL直接电泳，使用十分方便；电泳时500 bp的DNA片段量约为150 ng，显示亮带，其他条带的DNA量约为50 ng。

注意事项：

1．本产品可直接化冻混匀使用，无需加热。 

2．请及时更换电泳缓冲液并使用新制琼脂糖凝胶，以免影响电泳结果。 

3．DNA琼脂糖凝胶电泳时，凝胶的纯度对DNA条带的清晰度影响很大，因此尽量选用 质量好的琼脂糖，推荐凝胶浓度为2%-3%，电压4-8 v/cm。 

4．凝胶浓度与DNA片段的分离性能关系密切。凝胶浓度越大，对短片段DNA分离性能 越好；反之，凝胶浓度越小，越有利于长片段DNA的分离。 

5．当与大分子染料配合使用时，建议适当减少Marker的用量或加大染料的用量。  

使用方法：

取5 μL加入琼脂糖凝胶的加样孔中（如果加样孔较宽，大于6 mm时，须适当增加 上样量），进行电泳。

实验结果： 

1%琼脂糖凝胶电泳图(5 μl)

The 100 bp Ladder consists of 9 DNA fragments, namely 1500 bp, 1000 bp, 800 bp, 600 bp 500 bp, 400 bp, 300 bp, 200 bp, and 100 bp. This product contains DNA that already contains 1 x Loading Buffer Solution, can be taken as 5 μ L direct electrophoresis, very convenient to use; The amount of 500 bp DNA fragments during electrophoresis is approximately 150 ng,Displaying bright bands, the DNA content of other bands is approximately 50 ng.

Notes:
1. This product can be directly thawed and mixed for use without heating.
2. Please replace the electrophoresis buffer in time and use the newly prepared agarose gel to avoid affecting the electrophoresis results.
3. During DNA agarose gel electrophoresis, the purity of gel has a great impact on the clarity of DNA bands, so try to use high-quality agarose. The recommended concentration of gel is 2% -3%, and the voltage is 4-8 v/cm.
4. The concentration of gel is closely related to the separation performance of DNA fragments. The higher the concentration of gel, the better the separation performance of short DNA fragments; On the contrary, the smaller the gel concentration, the more conducive to the separation of long DNA fragments.
5. When used in combination with macromolecular dyes, it is recommended to appropriately reduce the amount of Marker or increase the amount of dye used.

Usage:
Take 5 μ L is added to the sample adding hole of agarose gel (if the sample adding hole is wider than 6 mm, the sample loading amount must be increased appropriately) for electrophoresis.

Experimental results:

1% agarose gel electrophoresis (5 μ L)