DNA Ladder (0.2-12kb, 12 bands, with Red)是一种即用型(ready for use)DNA分子量标准(DNA Molecular Weight Marker)，包含了12条双链DNA条带，可以满足各种常规DNA电泳分析的分子量参照需要。本DNALadder共含12条带，分别为200，500，800，1000，1500，2000，3000，4000，5000，6000，8000，12000bp (参见下图)。本DNALadder中2000bp条带亮度较高，是其它条带亮度的3倍，使辨别条带更加容易。本DNA Ladder中已添加了含有Red的DNA上样缓冲液，可以直接使用。染料Red电泳时的迁移速度快于100bp的双链线性DNA，这样就不会像溴酚蓝那样对小片段DNA的观察产生干扰。每孔上样5微升，即可观察到非常清楚的DNA条带(参见下图)。

注意事项：

如果使用强致癌性的溴化乙锭(EB)，须注意避免DNA Ladder在使用时被溴化乙锭污染，操作时一定要采取适当的防护措施。推荐使用无毒的EB升级换代产品NA-Red 、NA-Green 、Gel-Red 和Gel-Green 。电泳时请尽量使用未用过的电泳缓冲液和新配制的琼脂糖凝胶，以免影响电泳结果。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：

1.DNA琼脂糖凝胶电泳时直接加入5微升DNA Ladder至加样孔中，即可电泳。2.与溴酚蓝相比，Red电泳速度较快，一般其位置相当于50bp左右。

DNA Ladder (0.2-12kb, 12 bands, Red) is supplied in a ready-to-use format containing Red and 12 double-stranded DNA fragments ranging from 200bp to 12kb. It can meet the molecular weight reference needs for most regular DNA electrophoresis analysis.This DNA ladder contains 12 DNA fragments of 200, 500, 800, 1000, 1500, 2000, 3000, 4000, 5000, 6000, 8000 and 12000bp (see figure on the right).The intensity of 2000bp band is 3 times brighter than other bands, making it easier to identify.Red migrates faster than 100bp double-stranded linear DNA, so it will not interfere with the observation of small DNA fragments.Loading 5μl of DNA Ladder per lane is sufficient to visualize all fragments of the ladder.

Precautions：

If ethidium bromide (EB), a strong carcinogen, is used for DNA electrophoresis, please take care to avoid the contamination of this product by EB during frequent uses. We recommend using non-toxic, upgraded EB products such as NA-Red (, D0128, D0130), NA-Green , Gel-Red and Gel-Green .Please use new electrophoresis buffer and freshly prepared agarose gel for electrophoresis to ensure better DNA resolution .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.For DNA agarose gel electrophoresis, add 5μl of this product directly into an empty well on agarose gel.2.Red migrates similarly to 50bp DNA fragments.