品牌货号 产品名称 | 阿拉丁P128568 磷脂酶A2 来源于东部菱背响尾蛇毒液 |
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英文别名 | PLA2 | Lecithinase A | Phosphatidylcholine 2-acylhydrolase |
规格或纯度 | EnzymoPure™, ≥200 units/mg dry weight |
生化机理 | 水解齐聚物甘油磷脂的β-酯键。首选底物是磷脂酰胆碱、磷脂酰乙醇胺及其质原类似物。磷脂酰肌醇和磷脂酰丝氨酸也会被水解。对完整细胞膜中的磷脂具有攻击性。与单体底物相比,它对聚集底物具有较高的催化活性。 |
产品介绍 | Phospholipase A2 is a member of the class of heat-stable, calcium-dependent enzymes catalyzing the hydrolysis of the 2-acyl bond of 3-n-phosphoglycerides. The enzyme has a molecular weight of 30,000 daltons. Phospholipase A2 is activated by Ca2+. It is inhibited by zinc, barium, and manganese ions. Activity values for phospholipase A preparations which are derived from titrimetric assay procedures can be quite dependent on source and type of lecithin, its preparation as a substrate emulsion, other components of the reaction mixture, and the method and instrumentation used. A chromatographically purified, dialyzed, lyophilized powder. |
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