重组牛肠激酶是一种高纯度的重组牛肠激酶轻链片段。该酶经HPLC纯化，纯度高，特异性高，并且不含其他蛋白酶。肠激酶是一个特定的蛋白酶，它可以切割前面含有四个天冬氨酸的赖氨酸羧基端位点：天冬氨酸 - 天冬氨酸 - 天冬氨酸 - 天冬氨酸 - 赖氨酸。肠激酶可去除位于蛋白质N-末端的融合蛋白，以除去不需要的融合标签。特定的蛋白酶，切割前面含有四个天冬氨酸的赖氨酸羧基端位点：天冬氨酸 - 天冬氨酸 - 天冬氨酸 - 天冬氨酸 - 赖氨酸 (DDDDK)。不含其他蛋白酶,无非特异性切割。

Recombinant Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK).No any other contaminated proteases, no non-specific cutting sites. One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl,pH 8.0. Substrate: a special fusion protein