RNase H是特异性分解RNA-DNA杂交体中的RNA链的核糖核酸内切酶，该酶不能 消化单链或双链DNA。本产品可应用于DNA-RNA杂交体的检定，在cDNA第二链合成 时除去mRNA，去除杂交到poly (dT)上的mRNA的poly (A)尾等。
| R665576 | Component | 600 U | Storage | | R665576A | RNase H, 60 U/μL | 10 μL | -20℃. Avoid freeze/thaw cycle. | | R665576B | 5×RNase H Reaction Buffer | 300 μL | -20℃. Avoid freeze/thaw cycle. |
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活性定义 以poly (rA)·poly (dT) 为底物，在37°C条件下，20分钟内产生1 nmol酸可溶性物质 所需要的酶量定义为1个活性单位 (U)。 使用方法 1．以降解DNA-RNA杂合体中的RNA为例，配置20 μl反应体系。 
2．37°C反应30 min。 注意事项 本产品为热稳定的RNase H酶，在70°C或以上温度孵育10 min后仍具有RNase H活性。
RNase H is a ribonucleic acid endonuclease that specifically breaks down RNA strands in RNA DNA hybrids, and cannot digest single or double stranded DNA. This product can be used for the detection of DNA-RNA hybrids, removing mRNA during cDNA second strand synthesis, and removing the poly (A) tail of mRNA hybridized to poly (dT).
| R665576 | Component | 600 U | Storage | | R665576A | RNase H, 60 U/μL | 10 μL | -20℃. Avoid freeze/thaw cycle. | | R665576B | 5×RNase H Reaction Buffer | 300 μL | -20℃. Avoid freeze/thaw cycle. |
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Activity definition
The amount of enzyme required to produce 1 nmol of acid soluble substance within 20 minutes at 37 ° C using poly (rA) · poly (dT) as substrate is defined as 1 active unit (U).
Usage 1．Taking RNA degradation in DNA RNA heterozygotes as an example, configure 20 μ Reaction system. 
2．React at 2.37 ° C for 30 minutes. Note This product is a thermally stable RNase H enzyme that still exhibits RNase H activity after incubation at 70 ° C or above for 10 minutes.
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